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Wednesday, November 18, 2020 | History

4 edition of Epithelial & Stromal Cultures of the Human Prostate found in the catalog.

Epithelial & Stromal Cultures of the Human Prostate

Androgen Responsiveness & Control of Growth & Differentiation (Acta Biomedica Lovaniensia, 255)

by Karine Goossens

  • 73 Want to read
  • 10 Currently reading

Published by Leuven Univ Pr .
Written in English

  • Human reproduction, growth & development,
  • Urology & urogenital medicine,
  • Nursing - Oncology & Cancer,
  • Medical

  • The Physical Object
    Number of Pages154
    ID Numbers
    Open LibraryOL12845995M
    ISBN 109058672158
    ISBN 109789058672155

    Cell Culture. The human prostate cancer cell lines, ARCAP E, ARCaP M, the HSa bone stromal cells (ATCC, Manasss, VA) and the Pt-N or Pt-C human prostate stromal cell. Isolation and characterization of the human prostate cancer RFP-ARCaP cell lines has been reported. Red Fluorescent Protein- (RFP-) transfected cells were maintained in.   Prostate, Epithelium - Hyperplasia. Higher magnification of Figure 1. Proliferative epithelial cells have formed a thickened lining of the affected acini, and there is evidence of associated apoptosis (arrows) in a male F/N rat from a chronic study.   Using primary cultures of human uterine epithelial cells and stromal fibroblasts, we have demonstrated that the uterus is a potential site for productive .

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Epithelial & Stromal Cultures of the Human Prostate by Karine Goossens Download PDF EPUB FB2

Indicate that both heterologous human fibroblasts and mouse stromal cells are capable of permissively support-ing adult human prostate epithelial function.

Key words: epithelium, stroma, prostate, human, differentiated. Introduction One of the main problems in the investigation of prostatic disorders is the lack of easily established and.

Abstract. Well-established techniques are now in place to culture several of the major types of cells in the prostate. Epithelial cells with characteristics of basal and/or secretory luminal cells can be grown in vitro, as can stromal cells with properties of fibroblasts and/or smooth by:   Here we report the identification of several novel candidate prostate epithelial SC antigens in the human prostate gland.

Importantly, we found that the receptor tyrosine kinase KIT is located on ACexpressing epithelial cells positive for the KIT ligand SCF, Bcl-2, and CDCited by:   Culture conditions influence the phenotype of prostate organoids, and stromal regulation over epithelial cells is essential, yet a 3D model that includes prostate Epithelial & Stromal Cultures of the Human Prostate book has not been reported.

To address this need, we systematically optimized and characterized a 3D co-culture model that facilitates direct stroma-epithelial organoid by: Purpose. There is a lack of suitable in vitro models for the human prostate. To study stromal-epithelial interactions, we established stromal cells in cultures from benign and malignant prostate tissue that resemble more closely the in vivo conditions of the human by: Results.

Cells with a basal phenotype proliferated significantly in explant cultures, whereas luminal cells went into apoptosis. Results further show down-regulation in tissue cultures of the basal and hypothetical stem cell marker Bcl-2 in the majority of cells, except in rare putative epithelial stem cells.

The prostate with BPH exhibits enhanced growth not only in the epithelium but also in the stroma, and stromal‐epithelial interactions are thought to play an important role in BPH pathogenesis.

However, our understanding of the mechanisms of stromal‐epithelial interactions in the development and progression of BPH is very limited.

To reproduce the structural and functional differentiation of human prostatic acini in vivo, prostatic epithelial and stromal cells derived from human primary cultures were cocultured in Matrigel.

In the absence of stroma and serum, epithelial spheroids composed. The expression of the IL‐8 axis components was confined to the prostate luminal epithelial cells in both normal and BPH tissues. However, these components were elevated in BPH‐1 and primary explant cultures as compared to RWPE‐1, NHPrE1, and BHPrE1 cells.

patient-derived prostate cancer tissues implanted in immunocompromised mice and could serve as a precision medicine approach for each prostate cancer patient. Spheroid and organoid assays, representative of modern three-dimensional cultures, can replicate the conditions in human prostate tumors and the prostate organ itself as a miniature model.

Addition of Epithelial & Stromal Cultures of the Human Prostate book Primary Prostate Stromal Cells to 3D Culture of Human Benign Prostate Epithelial Cells Increases Organoid Branching. The method for co-culture using primary PrE and stromal cells was optimized by systematic modifications of cell density, matrix composition, culture medium, and plating format (Matrigel base layer or low-attachment plates).

The rat prostate is composed of a complex system of branching ducts which terminate proximally at the urethra. It has been recognized that epithelial cells lining the ducts respond differently to and.

The effects of human primary prostatic stromal cells on the migration and morphogenesis of human prostatic epithelial cells, derived from tumor or benign prostatic hyperplasia tissue, were studied using a three-dimensional coculture system.

Epithelial cells from tumor or benign tissue migrated efficiently into collagen gels populated with stromal cells from benign tissue. A human-derived prostate co-culture microtissue model using epithelial (RWPE-1) and stromal (WPMY-1) cell lines The development and normal function of prostate tissue depends on signalling interactions between stromal and epithelial compartments.

As in MG, mesenchymal‐epithelial interactions in the prostate begin during fetal periods, but continue into adulthood. The responsiveness of adult epithelial cells from various glands to stroma raises the possibility that carcinomas also may be regulated by connective tissue.

Our past research developing a molecular understanding of stromal and epithelial interaction in prostate growth and development. The establishment of human prostate cancer progression models addressing the reciprocal roles of stromal and epithelial interaction and the expression by tumor epithelium of androgen independent and metastatic.

Primary cell culture provides a model system that allows a broader spectrum of cell types from a greater number of patients to be studied, in the absence of artificially induced genetic mutations.

Nevertheless, primary prostate epithelial cell culture can be technically challenging, even for laboratories experienced in immortalized cell culture.

Prostate Cancer The Characterization of Epithelial and Stromal Subsets of Candidate Stem/Progenitor Cells in the Human Adult Prostate Jens A. Cedera,*, Linda Janssona, Roy A. Ehrnstro¨mb, Lars Ro¨nnstrandc, Per-Anders Abrahamssona aLund University, Department of Clinical Sciences, Division of Urological Research, University Hospital MAS, S 02 Malmo¨, Sweden.

Prostate cell culture methods were initially described several years ago, 37, 38 but in recent years methods of separating epithelial cells from the stromal tis 40 together with the.

Benign prostate epithelial-1 cell culture. Benign prostate epithelial (BPH-1) cells were kindly provided by the Australian Prostate Cancer Research Centre Queensland. The BPH-1 cell line was fluorescently labelled for tracking purposes as previously described. Briefly, the cell line was incubated overnight with an in-house-generated GFP.

Scientists engaged in prostate cancer research have been conducting experiments using two‐dimensional cultures of prostate cancer cell lines for decades. However, these. An immortalized human prostate stromal cell line (PS30) was previously established using recombinant retrovirus encoding human papillomavirus 16 gene products.

In this study, we further characterize this stromal cell line for its potential use in a stromal-epithelial coculture model for prostate.

Cells, cell cultures and reagents. The androgen-independent prostate cancer DU cell line was purchased from ATCC (Manassas, VA, USA). Normal prostate tissues for primary stromal culture were obtained from three consenting donors (a 23, and 40 years) who had no pre-existing prostate issues and died from other diseases at Shanghai Ninth People’s Hospital (Shanghai, China).

Fig. 1 Identification of prostate cell lineage markers in fresh (A) and cultured (B–F and I) human normal/benign prostate explants, and primary cell culture (G and H) response to hepatocyte growth factor (HGF). (A) The exocrine cell layer in fresh tissue was immunoreactive against the luminal cell marker prostate-specific antigen (PSA).

The myofibroblast stromal cell line, WPMY-1, was derived from stromal cells from the same peripheral zone of the histologically normal adult prostate, as that used for RWPE-1 cells (ATCC CRL).

Stromal cells were immortalized with SVlarge-T antigen gene, using a pRSTV plasmid construct. WPMY-1 stromal cells belong to a family of cell lines derived from the same prostate as the.

A Microfluidic-based model of a human prostate gland has been developed featuring a 3D co-culture of epithelial and stromal cells. The model was used to investigate the effects of normal stroma on normal luminal epithelial cell differentiation, and to measure the ability of cancerous epithelium to convert normal stroma to cancer stroma.

Human Prostate Stromal Cells (PrSC) are necessary for the normal growth and survival of epithelial cells in the prostate. A number of findings have suggested interactions between PrSC and the epithelium as well as importance of the extracellular matrix in prostate development and differentiation; alterations of these interactions may lead to.

Two prostate cancer cell-lines including androgen-independent DU and PC-3 and the PNT1A, normal human prostate epithelial cell-line, were used in co-culture design. Prostate cancer cells and normal prostate epithelial cells were allowed to grow on the same culture medium without direct cell-to-cell contact and to communicate with each other.

Using in vitro co-culture to simulate cancer–stromal interaction, this study revealed that prostate stromal cells could rescue LNCaP prostate cancer cells from serum starvation (Fig. Cultured alone, growth of the LNCaP cells was reduced by androgen deprivation, and all the LNCaP cells were killed by serum starvation (Fig.

4 B). Lonza's Human Prostate Cells are available from epithelial, smooth muscle, and stromal tissue. Each cell type has an optimized growth media for best results in culture. Prostate Epithelial Cells (PrEC) are guaranteed through 15 population doublings and stain positive for cytokeratin (clone ).

By using human cell co-culture models, we demonstrate that ASC induce epithelial-mesenchymal transition (EMT) in prostate cancer cells.

Our results for the first time demonstrate that ASC interaction renders cancer cells more migratory and resistant to docetaxel, cabazitaxel, and cisplatin chemotherapy.

Background: Benign prostatic hyperplasia (BPH) is an age-related disease characterized by nonmalignant abnormal growth of the prostate, which is also frequently associated with lower urinary tract symptoms. The prostate with BPH exhibits enhanced growth not only in the epithelium but also in the stroma, and stromal-epithelial interactions are thought to play an important role in BPH.

Compartmentalized expression of miR-1, miR, and miR in normal human prostate. Adjacent non-malignant regions from the same JHU radical prostatectomy cases (Fig. 1B) were then microdissected by xMD, to isolate the stromal and epithelial cells from normal prostate tissue (Figs 2A and S1).Specifically, stromal cells were isolated by anti-α-smooth muscle actin (α-SMA) IHC staining.

Cell Cultures. Human prostatic stromal cells were isolated from tissues obtained from three patients undergoing transurethral prostatectomy procedures for treatment of bladder neck obstruction secondary to BPH.

The diagnosis of BPH was confirmed by review of. The robust nature of our system is shown by the post-culture preservation of epithelial–stromal relationships, tumor tissue morphology, proliferation, and viability at all time points.

We show that cancer-related pathways such as the PI3K/Akt signaling pathway remain functionally active and stable in ex vivo culture both at the. Androgens are essential for the development, differentiation, growth, and function of the prostate through epithelial–stromal interactions.

However, androgen concentrations in the hypertrophic human prostate decrease significantly with age, suggesting an inverse correlation between androgen levels and proliferative diseases of the aging prostate. In elderly males, age- and/or androgen.

The role of stromal cells on the initiation and promotion of carcinogenesis has been studied over many years. This concept was pioneered from previous studies showing [] that tumor stroma, termed as CAF (cancer associated fibroblast), TAS (tumor associated stroma), or RS (reactive stroma), is often different from the normal stroma [].Normal prostate stromal cells play a protective role and.

Human Prostate Tissue Array. Human prostate tissue samples from 90 patients with diagnosed prostate carcinoma representing different Gleason scores were used to generate a tissue microarray. The microarray contained mm core samples, two from the peripheral tumor and one from the peripheral zone (PZ) away from the cancer involved area.

The effects of stromal and hormonal environment on the immortalized but nontumorigenic human prostatic epithelial cell line BPH-1 were investigated in an in vivo model. BPH-1 cells were recombined with rat urogenital sinus mesenchyme (UGM), and the tissue recombinants were grafted to the renal capsule of adult male athymic mouse hosts.

BPH-1 + UGM recombinants formed solid branching epithelial. The reactive stromal phenotype in the prostate is mechanistically important for understanding prostate cancer progression and may be a target for prevention. We mimicked the interaction of endocrine, paracrine, and immune factors on induced androgen metabolism in prostate stroma by coculturing human primary prostate stromal cells and LAPC.

The objective of the current study was to determine whether dutasteride would induce apoptosis in a range of prostate epithelial cell lines and primary cultures. METHODS. Both human prostate androgen‐sensitive cell lines (PwR‐1E, PNT‐2, LNCaP, and PC3[AR2]) and an androgen‐independent cell line (PC‐3) were grown to confluence.Background: Benign prostatic hyperplasia (BPH) is an extremely common disease of older men characterized by increased growth of prostatic epithelial and stromal cells.

Previously we showed that senescent epithelial cells accumulate in the prostate of aging men and .Stromal cells strictly modulate the differentiation of the normal prostate epithelium.

In benign prostatic hyperplasia (BPH) tissue, the ratio of stromal to epithelial cells reaches a ratio. In this study, we evaluated the effects of crossover conditioned media (CM) of stromal and epithelial pro .